The Determination of Vitamin C with 2, 4, Dinitrophenylhydrazine

Determination of Formaldehyde in Human Tissue through Derivatization with 2 , 4 - Dinitrophenylhydrazine by Square Wave Polarography Method

The polarographic behaviour of formaldehyde (FA) was studied using cyclic and square wave polarography (SWP) methods. FA was derivatized with 2.4-dinitrophenylhydrazine (DNPH). The influence of several variables (including nature of the buffer, pH, concentration, scan rate, drop size, etc.) was examined in SWP method for FA. The best SWP responses were obtained in acetate buffer pH 5.0. The pea...

Determination of vitamin C in iranian citrus fruits

A simplified 2, 4-dinitrophenylhydrazine assay for Flavonoids and its comparison with a Standard flavonoid assay

A simplified version of the 2, 4-DNPH assay for flavonoids has been improvised. The assay includes incubating test sample with DNPH in a 4.5 mL reaction volume in hot water at about 80 C containing 2 mL methanol at about 0.2 N HCl, cooling samples to room temperature, adding 3 mL 1M NaOH, and monitoring red color at 470 nm at 10-15 minutes. The reagent has been found capable of assaying both fl...

Modified method for the determination of pyruvic acid with dinitrophenylhydrazine in the assessment of onion pungency

Modifications to the commonly used Schwimmer and Weston procedure for determining pyruvic acid in onions are proposed. These modifications involve changes in the concentrations and volumes of the reagents and the use of 515 rather than 420 nm as the wavelength for measurements. These changes improve both the linearity and the sensitivity of the assay and eliminate interference from coloured mat...

determination of maximal singularity free zones in the workspace of parallel manipulator

due to the limiting workspace of parallel manipulator and regarding to finding the trajectory planning of singularity free at workspace is difficult, so finding a best solution that can develop a technique to determine the singularity-free zones in the workspace of parallel manipulators is highly important. in this thesis a simple and new technique are presented to determine the maximal singula...